Abcam, ab99297, Recombinant Human UAP56 protein

Size: 50µg / 250µg
Recombinant Human UAP56 protein is a Human Full Length protein, in the 1 to 428 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q13838,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UAP56 also known as DDX39B or SUB2 is an ATP-dependent RNA helicase with a molecular mass of approximately 46 kDa. It plays a significant role in RNA processing by unwinding RNA duplexes. UAP56 is found throughout the nucleus and functions in various cell types with high expression levels in actively dividing cells. It binds RNA substrates using ATP hydrolysis facilitating movement along RNA strands and this action is vital for splicing and mRNA export from the nucleus to the cytoplasm.
Biological function summary
This protein contributes to the assembly of the spliceosome complex which is essential for pre-mRNA splicing. By interacting with other splicing factors UAP56 ensures accurate removal of introns and the joining of exons in the mRNA. Its function is also necessary for the proper export of mRNA through the nuclear pore complex. Through these activities UAP56 supports gene expression by ensuring that only mature mRNA is available for translation.
Pathways
UAP56 operates in the mRNA processing and transport pathways. In the mRNA export pathway it collaborates with other proteins like Aly/REF and the TREX complex facilitating the translocation of mRNA from the nucleus to the cytoplasm. In the splicing pathway UAP56 interacts with small nuclear ribonucleoproteins (snRNPs) to ensure correct splicing. These interactions help maintain the efficiency and fidelity of gene expression.
Defects in UAP56 or its interactions may contribute to cancer and neurodegenerative diseases. In cancer abnormal mRNA export and splicing can lead to the expression of oncogenes or the loss of tumor suppressors implicating UAP56 in tumor progression. In neurodegenerative disorders UAP56 dysfunction might result in improper mRNA processing leading to the accumulation of misfolded proteins. Its role is related to proteins such as TDP-43 which is involved in neurodegenerative pathologies.