Abcam, ab99326, Recombinant Human SAHH protein

Size: 100µg / 500µg
Recombinant Human SAHH protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, WB.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P23526,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.4Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Product details:
This product was previously labelled as S adenosylhomocysteine hydrolase

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.
Biological function summary
S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.
Pathways
S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.
S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.