Abcam, ab99436, Anti-NPAT antibody

Size: 100µg
Rabbit Polyclonal NPAT antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NPAT aa 700-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NPAT aa 700-750. The exact immunogen used to generate this antibody is proprietary information.Q14207

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NPAT also known as nuclear protein ataxia-telangiectasia locus is a protein that weighs approximately 220 kDa. It is widely present in various tissues with a high expression in proliferating cells. NPAT participates in cell cycle regulation and links directly to the control of histone gene expression during the G1/S phase transition. Its presence in the nuclear matrix further highlights its integrative role in gene regulation.
Biological function summary
NPAT plays an important role in the cell cycle's advancement by associating with the MMB (Myb-MuvB) complex. This interaction signifies its importance in DNA replication and cell cycle progression. NPAT activates transcription upon phosphorylation by CDK2/cyclin E influencing S-phase entry. It acts as a transcriptional regulator for histone gene expression necessary for chromatin structure and genome stability.
Pathways
NPAT integrates into the cyclin/CDK regulatory pathway governing cell cycle progression and is related to histone gene clusters regulation. The interaction with CDK2 illustrates its part in the regulation of cell proliferation. NPAT also associates with the E2F cell cycle pathway further influencing gene transcription important for DNA replication.
NPAT relates to cancer and ataxia-telangiectasia. These connections highlight its importance in cell cycle control disruption which can lead to oncogenesis. NPAT's relationship with the ATM protein emerges in the context of ataxia-telangiectasia suggesting a link between DNA damage response defects and neurological disorders. These associations reinforce NPAT’s involvement in maintaining cellular health and genomic integrity.