Abcam, ab99979, Human FGF basic ELISA Kit (FGF2)

Size: 1 x 96Tests
Human FGF basic ELISA Kit (FGF2) is a sandwich ELISA designed to quantify Human FGF basic (FGF2) with a sensitivity of 50 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 102.4 - 10000 pg/mL - Cited in over 10 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 50 pg/mL,
Range:102.4 - 10000 pg/mL,
Assay Platform:Microplate

Product details:
Human FGF basic ELISA Kit (FGF2) ab99979 is a sandwich ELISA to measure Human FGF basic (FGF2) in serum, plasma, cell culture supernatant, cell lysate with a sensitivity of 50 pg/ml.
How the assay works
This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human FGF basic ELISA Kit (FGF2) ab99979 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FGF basic also known as FGF-2 or bFGF is a member of the fibroblast growth factor family. This protein is approximately 18 kDa in mass and is expressed in various tissues including the brain skin and vascular endothelium. Its alternate forms include FGF-2 and FGF-basic which serve similar functional roles. FGF basic acts mechanically by binding to fibroblast growth factor receptors (FGFRs) initiating a cascade of intracellular signaling that leads to cell growth and differentiation.
Biological function summary
Fibroblast growth factor 2 is important in development and wound healing. It does not form part of a larger complex but interacts directly with receptors on the cell surface to activate downstream signaling pathways. FGF basic plays an important role in angiogenesis facilitating the growth of new blood vessels. Additionally this protein supports the proliferation of a wide range of cell types including fibroblasts and endothelial cells.
Pathways
Fibroblast growth factor 2 participates mainly in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for controlling cell division and survival. In the MAPK/ERK pathway FGF basic's interaction with FGFR involves proteins such as Ras and Raf. Similarly in the PI3K/AKT pathway it engages with proteins including PI3K and Akt coordinating various cellular responses to environmental stimuli.
FGF basic links to various cancers and cardiovascular diseases. In cancer abnormal FGF basic signaling can lead to uncontrolled cell growth where it interacts with oncogenic proteins like Ras. In cardiovascular disorders altered FGF basic expression affects vascular integrity often in conjunction with other proteins like VEGF contributing to pathological conditions such as atherosclerosis.