Agilent, 102905-100, Seahorse XFe96 Spheroid FluxPak

Seahorse XFe96 Spheroid FluxPak. Measure functional metabolism in 3 dimensions from in vitro spheroids. Each Seahorse XFe96 Spheroid FluxPak contains: 6 Seahorse XFe96 Spheroid Microplates, 6 XFe96 sensor cartridges, and 1 bottle of Seahorse XF Calibrant Solution 500mL.

Specifications:
Assay Running Volume: 200-250 µL/well
Assay Wells: 96
Biological Sample Type: Spheroid
Bundle Contents: 1 500ml bottle of calibrant6 XFe96 Spheroid Microplates6 XFe96 sensor cartridges
Cell Dimension: 3D
For Use With: Seahorse XF Pro AnalyzerSeahorse XFe96 Analyzer
Injection Ports per Well: 4 per well
Microchamber Height: 250
Microchamber Volume: 1.88 µL
Plate Surface Treatment: High Binding
Sample Requirements: 1-100 cells/well
Shipping Condition: Ambient Temperature
Volume per Port Injection: 30 µL
Well Volume: 250 µL

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This specialized reagent kit is designed for high-throughput metabolic analysis of 3D spheroid and microtissue models using the XFe96 Analyzer platform. It enables reliable measurement of cellular bioenergetics, specifically oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), from multicellular aggregates cultured in 96-well format. Researchers can assess mitochondrial function, glycolysis, and metabolic reprogramming in tumor spheroids, organoids, and stem cell-derived 3D structures, making it ideal for cancer research, drug discovery, toxicity screening, and tissue engineering studies. The kit includes individually packaged sensor cartridges and utility plates optimized for compatibility with the Agilent Seahorse XFe96 Analyzer. For optimal results, it is recommended to use this kit in conjunction with Seahorse XF Spheroid Microplates and complementary assay media or reagents tailored to specific metabolic pathways. With its streamlined workflow, the kit facilitates reproducible and sensitive measurement of metabolic flux in complex biological samples, enabling comprehensive functional profiling and direct comparison among 3D cultures. It supports robust assay performance and is an essential component for laboratories seeking insights into cellular metabolism in physiologically relevant three-dimensional systems.