Agilent, 103015-100, Seahorse XF Cell Mito Stress Test Kit

Seahorse XF Cell Mito Stress Test Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of oligomycin, FCCP, and rotenone/antimycin A. For use with Seahorse XFe/XF96 and XF/XF24 analyzers.

Specifications:
Assay Wells: 6 x 96-well or 24-well assays
Assays per Kit: 6
For Use With: Seahorse XF Flex AnalyzerSeahorse XF Pro AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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The Seahorse XF Cell Mito Stress Test Kit enables comprehensive evaluation of mitochondrial function in live cells. Designed for use with Agilent Seahorse XF Analyzers, this assay kit allows researchers to rapidly and precisely quantify key bioenergetic parameters, including basal respiration, ATP production, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration. By delivering a sequential addition of specific mitochondrial inhibitors and uncouplers, the kit facilitates real-time analysis of energy metabolism, supporting studies in metabolic profiling, drug screening, cell signaling, and disease modeling. The straightforward protocol and ready-to-use reagents ensure consistent and reproducible results across various cell types and experimental designs. Typical application areas include oncology, immunology, neuroscience, and stem cell research where insights into mitochondrial function are critical. The kit is optimized for use with the entire line of Seahorse XF platforms, including the XF HS Mini, XF96, and XF24 analyzers. It can also be used in combination with Seahorse XF cell culture microplates and other cell metabolic assays for an integrated approach to cellular bioenergetics. This solution streamlines the evaluation of mitochondrial health, enabling laboratories to unlock deeper insights into cellular metabolism and its implications in health and disease.