Agilent, 103344-100, Seahorse XF Glycolytic Rate Assay Kit

Seahorse XF Glycolytic Rate Assay Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of rotenone/antimycin A and 2-deoxy-D-glucose. For use with XFe/XF96 and XFe24 analyzers. Not compatible with XF24 and XF24-3 analyzers. XF pheno-red free media with low buffer capacity (103575-100 or 103576-100) are required to perform the assay.

Specifications:
Assay Wells: 6 x 96-well or 24-well assays
Assays per Kit: 6
For Use With: Seahorse XF Pro AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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The Seahorse XF Glycolytic Rate Assay Kit provides researchers with a robust solution for quantifying glycolytic activity in live cell populations. Using real-time extracellular flux analysis, this kit enables simultaneous measurement of key metabolic parameters, including proton efflux and glycolytic proton efflux rates. The assay is ideal for applications in cancer metabolism, immunology, stem cell biology, and drug discovery, where understanding glycolytic flux is essential. Designed for compatibility with Agilent Seahorse XF Analyzers, including XFe and XFp models, it offers streamlined workflow integration and reliable performance. The kit supplies all necessary optimized reagents and calibrations for accurate detection and analysis. Researchers benefit from comprehensive data on mitochondrial and glycolytic function, facilitating the study of energy pathway preferences, metabolic shifts, and cellular responses to substrates or inhibitors. Whether investigating cellular responses to hypoxia, evaluating metabolic reprogramming, or screening compounds that modulate glycolysis, this kit delivers reproducible results with high throughput capability. It is also well-suited for use alongside other Seahorse XF assay kits to generate a complete metabolic profile. The product thus enhances the precision and depth of metabolic research across a range of biological and biomedical fields.