Agilent, 103575-100, Seahorse XF DMEM medium, pH 7.4, 500 mL.

Seahorse XF DMEM medium, pH 7.4, 500 mL. This is a DMEM-based medium and does not contain phenol red, bicarbonate, glucose, pyruvate, or glutamine. It contains 5 mM HEPES, and the pH of this medium is pre-adjusted to 7.4. To ensure a proper final pH in assay media, Seahorse XF supplements must be used at recommended concentration range. Check Seahorse XF media and buffer selection guide for more details. This medium is validated for use with the Seahorse Real-Time ATP rate assay and the glycolytic rate assay. It is also recommended to use for all Seahorse XF assays.

Specifications:
For Use With: Seahorse XF24 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 AnalyzerSeahorse XFp Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Refrigeration required, 2-8°C
Volume: 500 mL

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
Agilent Seahorse XF DMEM medium pH 7.4 is a specially formulated cell culture medium optimized for use in metabolic flux analysis. Supplied in a 500 mL bottle, this pre-buffered DMEM-based medium is designed to support accurate and reproducible measurements of cellular bioenergetics, including mitochondrial respiration and glycolysis. The formulation provides consistent baseline conditions and minimizes assay variability during Seahorse XF Analyzer experiments, making it ideal for studies requiring precise control of pH and nutrients.

This medium is recommended for preparing cells prior to running Seahorse XF assays, as well as for use as an assay medium during the actual measurements. It is suitable for a wide range of adherent mammalian cell types and is fully compatible with the Agilent Seahorse XF family of analyzers, including the XFe and XF HS Mini models. Researchers can use this medium in applications such as profiling cellular energy metabolism, investigating mitochondrial function, or screening drug candidates for metabolic effects. For optimal performance, it can be supplemented with user-specific substrates like glucose, glutamine, and pyruvate as needed. Use with Seahorse XF cell culture microplates and fluxpaks is recommended for streamlined workflow.