Agilent, 103592-100, Seahorse XF Real-Time ATP Rate Assay Kit

Seahorse XF Real-Time ATP Rate Assay Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of oligomycin and rotenone/antimycin A. For use with Seahorse XFe/XF96 and XFe24 analyzers.

Specifications:
Assay Wells: 6 x 96-well or 24-well assays
Assays per Kit: 6
For Use With: Seahorse XF Pro AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 Analyzer
Shipping Condition: Ambient Temperature
Storage Temp Conditions: Store at room temperature

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The Seahorse XF Real-Time ATP Rate Assay Kit enables precise quantification of cellular ATP production rates in live cells by differentiating between mitochondrial and glycolytic pathways. Designed for use with Agilent Seahorse XF Analyzers, this kit delivers real-time, dynamic measurement of energy metabolism, allowing researchers to monitor shifts in bioenergetic phenotypes under various experimental conditions. The assay provides an integrated workflow from assay setup to data analysis and is particularly valuable for applications in drug discovery, mitochondrial function studies, metabolic screening, cancer metabolism, and immunometabolism research. The kit includes all necessary reagents and optimized protocols to ensure robust, reproducible results and is compatible with a variety of cell types, including primary cells and adherent or suspension cell lines. When combined with other Seahorse XF assays, such as the Cell Mito Stress Test or Glycolysis Stress Test, the ATP Rate Assay provides comprehensive insights into cellular respiration and energy production. Supporting intuitive data analysis via Wave software, this kit streamlines metabolic profiling and supports high-throughput studies, making it a critical tool for laboratories seeking to unravel bioenergetic mechanisms or assess mitochondrial dysfunction. The system is suitable for use with XF24, XF96, XFp, and other supported Agilent Seahorse XF platforms.