Agilent, 103798-100, Seahorse XFe96/XF Pro PDL FluxPak Mini

Seahorse XFe96/XF Pro PDL FluxPak Mini. For use with XFe96 and XF Pro Analyzer. Seahorse XFe96/XF Pro PDL FluxPak Mini includes 6 XFe96/XF Pro sensor cartridges, 6 XFe96/XF Pro PDL Cell Culture Microplates, and 1 bottle of Seahorse XF Calibrant Solution 500mL.

Specifications:
Assay Running Volume: 200-250 µL/well
Assay Wells: 96
Biological Sample Type: AdherentIsolated MitochondriaMonolayerSuspension
Bundle Contents: 1 500ml bottle of calibrant1 pair of XF Loading Guide6 XFe96/XF Pro PDL Cell Culture Microplates6 XFe96/XF Pro sensor cartridges
Cell Dimension: 2D
For Use With: Seahorse XF Pro AnalyzerSeahorse XFe96 Analyzer
Injection Ports per Well: 4 per well
Microchamber Height: 200 µm
Microchamber Volume: 2.28 µL
Plate Surface Treatment: Irradiated
Sample Requirements: 5000 - 200000 cells/well
Shipping Condition: Ambient Temperature
Well Volume: 200-250 µL/well

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The Seahorse XFe96/XF Pro PDL FluxPak Mini is a ready-to-use, pre-coated plate designed to streamline the setup of high-throughput metabolic flux assays. Coated with poly-D-lysine, this mini format accessory supports optimal cell adherence, especially for weakly attached or suspension cell types, enhancing the consistency of bioenergetics measurements. It is ideally suited for laboratories conducting mitochondrial respiration and glycolytic function assessments across various research fields, including cancer metabolism, immunology, neuroscience, and toxicology. This consumable is compatible with Agilent Seahorse XFe96 and XF Pro analyzers, enabling seamless integration with established assay protocols such as the Seahorse XF Cell Mito Stress Test, Glycolysis Stress Test, and Real-Time ATP Rate Assay. Its format minimizes reagent use and sample waste, making it cost-effective for pilot studies, assay development, or limited sample analysis. The PDL-coated surface ensures reproducible results and improved cell retention throughout assay incubation and washing steps. For researchers requiring larger-scale throughput, full-size FluxPak options are available. This product’s reliability and ease of use facilitate robust and reproducible metabolic data generation, supporting diverse biochemical and molecular research endeavors.