Agilent, 5065-4460, ZORBAX 300SB-C8 3.5µm, 0.3 x 150 mm

ZORBAX 300SB-C8 3.5µm, 0.3 x 150 mm. A reversed-phase C8 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C8
Hardware: SS
Inner Diameter (ID): 0.3 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: 300SB-C8
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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Designed for robust and reliable reversed-phase chromatography, this high-performance analytical column features a stable C8 bonded phase and a 3.5-micron particle size, delivering efficient separation of peptides, small proteins, and hydrophobic biomolecules. Its 0.3 mm internal diameter and 150 mm length make it ideal for narrow bore and capillary LC applications, including proteomics, peptide mapping, and pharmaceutical research where sample volume is limited and resolution is critical.

The stable bonded silica support allows use across a wide pH range, ensuring reproducible results with minimal baseline drift and peak tailing. Its advanced endcapping reduces silanol activity, preserving analyte integrity and improving peak shapes for both basic and acidic compounds. The column is compatible with Agilent 1100, 1200, and 1260 series LC and LCMS systems, and can be readily integrated into other standard HPLC platforms supporting capillary formats.

Optimal for both qualitative and quantitative analyses, it supports workflows in biotechnology, drug discovery, and quality control settings. This product complements the full range of Agilent ZORBAX StableBond columns and is a versatile choice for researchers seeking high resolution, reliability, and extended column lifetime for complex biological or pharmaceutical separations.