Agilent, 5190-2455, Agilent Bio WCX, NP10, 4.6 x 250 mm, PEEK

Agilent Bio WCX, NP10, 4.6 x 250 mm, PEEK. A weak cation exchange HPLC column with nonporous particles. Particle size is 10 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio WCX
Column Material: PEEK
Hardware: PEEK
Inner Diameter (ID): 4.6 mm
LC Platform: BioInert
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WCX, NP10, 4.6 x 250 mm, PEEK is a weak cation exchange chromatography column engineered for high-resolution separation of proteins, peptides, and other biomolecules based on charge differences. Featuring a 4.6 mm internal diameter and a 250 mm length, this column utilizes NP10 resin technology, offering optimal binding capacity and efficient elution profiles for analytical and preparative workflows. The column housing is constructed from PEEK, ensuring chemical resistance and bio-inertness, which is essential for minimizing sample adsorption and contamination.

Ideal for characterizing biotherapeutics, such as monoclonal antibodies, bispecifics, or protein variants, it supports detailed charge variant analysis and quality control assessments in pharmaceutical and academic research. It is compatible with a wide range of Agilent liquid chromatography systems, including the 1260 Infinity and 1290 Infinity II LC platforms, as well as other standard high-performance liquid chromatography instruments.

This column is well-suited for integration with UV, DAD, or MS detection, accommodating diverse analytical demands. Agilent also offers complementary Bio SAX and reversed-phase columns, enabling comprehensive protein characterization strategies. The solution provides reliable and reproducible results for complex protein profiling, supporting robust workflow execution in regulated and research laboratories.