Agilent, 675775-901, AdvanceBio MS Spent Media 100 Å, 2.1 x 100 mm

AdvanceBio MS Spent Media 100 Å, 2.1 x 100 mm. Zwitterionic HILIC column that delivers fast, sensitive, and reproducible separations of underivatized amino acids and polar metabolites found in bioprocessor cell culture media for LC-MS detection.

Specifications:
Brand: AdvanceBio MS Spent Media
Hardware: PEEK Lined SS
Inner Diameter (ID): 2.1 mm
LC Platform: BioInert
Length: 100 mm
Maximum Temperature: 80 °C
Part Number Application: Spent Media Analysis
Part Number Application Domain: Biopharma
Particle Size: 2.7 µm
Particle Type: Superficially Porous
Phase: HILIC-Z
Pore Size: 120 Å
Pressure Rating: 600 bar
Separation Mode: HILIC
Shipping Solvent: Acetonitrile/Water
Technique: LC & LC/MS
UNSPSC Code: 41115709
pH Range: 3-11

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The AdvanceBio MS Spent Media 100 Å, 2.1 x 100 mm column is engineered specifically for high-performance analysis of spent cell culture media in biopharmaceutical research and process development. This reversed-phase column features a 100 Angstrom pore size, optimized particle technology, and dimensions ideal for sensitive liquid chromatography-mass spectrometry (LC-MS) workflows. Designed to enable rapid, reproducible separation of a broad range of analytes, including small polar metabolites, host cell proteins, and process-related impurities, it streamlines profiling of complex culture media samples.

Ideal for applications in bioprocess monitoring, media optimization, and characterization of cell culture growth environments, this column supports reliable quantitation and identification of process-related components. Its robust stationary phase chemistry ensures low carryover and high recovery, making it suitable for routine laboratory protocols as well as critical quality control testing.

Fully compatible with Agilent’s LC and LC-MS/MS systems, as well as other major chromatography platforms, the column integrates seamlessly into existing analytical setups. Pairing with Agilent’s AdvanceBio sample preparation kits and reagents provides optimal performance for biotherapeutic development workflows. This product is a vital tool for biopharma researchers seeking precise, reproducible data on spent media composition and process-related impurities.