Agilent, GK80021, AdvanceBio Sialidase S, His-tagged

AdvanceBio Sialidase S, His-tagged. Enzyme releases α(2-3)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Replaces Sialidase S (GK80020). Includes 5x reaction buffer B [250 mM sodium phosphate pH 6.0].

Specifications:
Enzyme Formulation: Supplied lyophilized. 20 mM Tris-HCl, 25 mM NaCl (pH 7.5) upon reconstitution as indicated
Enzyme Source: AdvanceBio Sialidase S. Recombinant from Streptococcus pneumoniae, expressed in E. coli. 6xHis-tagged. Sialidases are also known as neuraminidases.
Enzyme Specificity: This enzyme preferentially releases a(2-3)-linked N-acetylneuraminic acid (Neu5Ac, NANA) from complex carbohydrates. Sialidase S is also active against a(2-3)-linked N-glycolyneuraminic acid [1] (Neu5Gc, NGNA), although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. Although the a(2-3)-linkage is preferred, Sialidase S (also known as NanB) is also capable of cleaving a(2-6)-linked sialic acid [3], particularly at higher concentrations. Adding an excess of this enzyme can lead to some cleavage of a(2-6)-linked sialic acid. Using GK80021 as directed in the certificate of analysis reduces the likelihood of these effects. Users may wish to optimize the enzyme concentration needed for specific proteins. May be used for exoglycosidase sequencing with Sialidase A (GK80040), which has a broader specificity, releasing a(2-3,6,8,9)-linked sialic acids. Sialidases are also known as neuraminidases.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5
Molecular Weight: ~75 kD
Unit: 3 U

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AdvanceBio Sialidase S, His-tagged is a recombinant enzyme specifically designed for efficient cleavage of sialic acid residues from glycoproteins, glycolipids, and oligosaccharides. Its C-terminal His-tag enables convenient purification and immobilization compatibility for streamlined workflows. This high-purity sialidase optimally removes terminal sialic acids without affecting the underlying glycan structure, making it an essential tool for comprehensive glycan profiling, glycoprotein characterization, and biotherapeutic quality control.

The enzyme exhibits broad substrate specificity, ensuring effective desialylation across diverse biological samples, including monoclonal antibodies and serum glycoproteins. Researchers in the fields of biopharmaceutical development, structural biology, and biomarker discovery benefit from its robust performance in automated and manual sample preparation protocols.

AdvanceBio Sialidase S, His-tagged integrates seamlessly with Agilent’s AdvanceBio Glycan Mapping columns and LC-MS systems, facilitating high-sensitivity glycan analysis. It is compatible with a range of analytical platforms and workflows for released N-glycan and O-glycan studies as well as mass spectrometry-based characterization. The product is supplied in a ready-to-use format, enabling reproducible results and minimizing sample preparation time for reliable data generation in glycoscience research and quality assurance environments.