Agilent, GK80030, AdvanceBio Sialidase C, 1 U

AdvanceBio Sialidase C, 1 U. Enzyme releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Includes 5x reaction buffer B [250 mM sodium phosphate pH 6.0].

Specifications:
Concentration: 10 U/mL
Enzyme Formulation: 20 mM Tris HCl pH 7.5, containing 25 mM NaCl
Enzyme Source: Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24. Sialidases are also known as neuraminidases.
Enzyme Specific Activity: ≥40 units/mg
Enzyme Specificity: This enzyme releases a2-3, and a2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc a2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. Sialidase A (GK80040) is recommended for this application.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5
Molecular Weight: ~41,000 daltons
Unit: 1 U
Volume: 100 µL
pH Optimum: 6

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AdvanceBio Sialidase C, 1 U facilitates the specific enzymatic removal of terminal sialic acid residues from glycoproteins and glycolipids, enabling detailed glycan structure analysis. This highly purified enzyme is optimized for superior activity and consistency, making it an essential tool for glycoprotein characterization, especially in biopharmaceutical and academic research workflows focused on glycosylation profiling. Researchers utilize this sialidase for both N-linked and O-linked glycan studies in applications such as quality control of therapeutic antibodies, biomarker discovery, and structure-function analysis. The product performs effectively under a range of reaction conditions and can be used as part of comprehensive glycan analysis workflows, including mass spectrometry, HPLC, and capillary electrophoresis. It is fully compatible with Agilent AdvanceBio Glycan Mapping columns, kits, and workflow solutions, allowing integration into established analytical pipelines for streamlined and reproducible results. AdvanceBio Sialidase C is also suitable for use with workflows employing Agilent LC systems and other leading instruments for glycoproteomics. Quality-assured and rigorously tested, this enzyme offers reliability and high specificity, making it ideal for scientists requiring precise and efficient desialylation when analyzing complex glycoprotein mixtures.