Agilent, GK80040, AdvanceBio Sialidase A, 1 U

AdvanceBio Sialidase A, 1 U. Enzyme releases α(2,3)-, α(2,6)-, α(2,8)-, and α(2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Also capable of releasing N-glycolylneuraminic acid. Incudes 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0] which when diluted gives 50 mM sodium phosphate pH 6.0.

Specifications:
Concentration: 5 U/mL
Enzyme Formulation: 20 mM Tris HCl pH 7.5, containing 25 mM NaCl.
Enzyme Source: Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases.
Enzyme Specific Activity: 40 units/mg
Enzyme Specificity: The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5.
Molecular Weight: ~88 kD
Unit: 1 U
Volume: 200 µL
pH Optimum: 6

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AdvanceBio Sialidase A is a high-quality enzyme preparation purified for robust and reliable removal of terminal sialic acid residues from glycoproteins, glycolipids, and other sialylated biomolecules. Supplied at a defined activity of 1 unit, this product enables precise enzymatic digestion for detailed glycan structure analysis. Its specificity makes it exceptionally suited for researchers involved in glycoprotein characterization, biopharmaceutical development, and quality control processes requiring accurate sialic acid profiling.

Widely applied in workflows such as glycan mapping, biomarker discovery, and therapeutic antibody assessment, this enzyme is ideal for sample preparation prior to chromatographic or mass spectrometric analyses. AdvanceBio Sialidase A integrates seamlessly with Agilent’s suite of AdvanceBio Glycan Mapping UHPLC columns and AdvanceBio RP columns, supporting comprehensive glycan analysis from release through detection.

The high purity and activity of the enzyme ensure minimal background interference and reproducible results across diverse biological samples. Its compatibility with Agilent HPLC, LC-MS systems, and related sample handling accessories streamlines analytical processes in regulated and research laboratory settings. This product is critical for researchers seeking confidence in their glycan analysis, facilitating efficient, accurate, and consistent enzymatic deglycosylation.