Agilent, GK80043, AdvanceBio Sialidase A, Sampler Kit

AdvanceBio Sialidase A, Sampler Kit. Contains lower MW sialidase A isoforms for challenging sialidase digests. This sampler kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.

Each enzyme vial contains 200 mU in 40 µl. Also included is one vial 5x reaction buffer (1 mL). These products are available for purchase separately in 1-Unit pack sizes under the individual product codes GK80040 (88 kDa), GK80046 (66 kDa) and GK80045 (51 kDa).

Sialidases are also known as neuraminidases. Specifications: Concentration: 5 U/mL Enzyme Source: Recombinant gene from Arthrobacter ureafaciens expressed in E. coli. Sialidases are also known as neuraminidases. Enzyme Specificity: The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase. Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5 pH Optimum: 6 **********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.********** The AdvanceBio Sialidase A Sampler Kit from Agilent is designed for researchers specializing in glycoprotein structure and functional analysis. This comprehensive kit provides highly purified sialidase enzyme, facilitating the efficient and selective removal of terminal sialic acids from glycoproteins, glycolipids, and oligosaccharides. Its precise activity supports the detailed study of glycan heterogeneity, enabling accurate profiling of glycosylation patterns in complex biological samples. Ideal for biopharmaceutical, academic, and clinical laboratories, the kit streamlines glycan characterization workflows, including sample preparation for LC, LC/MS, and HPLC analyses. It is compatible with Agilent’s suite of AdvanceBio columns and instrumentation, such as 1260 Infinity and 1290 Infinity LC systems, providing seamless integration into existing analytical platforms. Researchers working in biosimilar development, quality control, and disease biomarker identification benefit from its reliability and consistency. This sampler kit accelerates method development, validation, and routine characterization, reducing experimental variability and enhancing data reproducibility. It is also compatible with other enzymes and analytical reagents in the AdvanceBio portfolio, offering flexibility for customized workflows targeting a range of glycosylation studies. The kit supports high-throughput settings and delivers dependable performance for advanced glycan research.