Agilent, GK80060, AdvanceBio Sialidase V, Recombinant

AdvanceBio Sialidase V, Recombinant

Specifications:
Concentration: 5 U/mL
Enzyme Applications: Glycan analysis, glycan digestion, sialic acid analysis, sialic acid removal, desialylation
Enzyme Formulation: Sterile-filtered solution in 50 mM sodium acetate, 150 mM NaCl, pH 5.5
Enzyme Source: Recombinant, expressed in E. coli, from Vibrio cholerae
Enzyme Specificity: Cleaves all nonreducing terminal α(2-3,6,8)-linked-sialic acid fromoligosaccharides, glycoproteins, and complex carbohydrates
Enzyme Unit Definition: One unit of AdvanceBio Sialidase V is defined as theamount of enzyme required to catalyze the releaseof 1 μmol of methylumbelliferone from MU-NANA[2'-(4-methylumbelliferyl)- α-d-N-acetylneuraminic acid] perminute at pH 5.5 and 37 °C
Storage Temp Conditions: Refrigeration required, 2-8°C
UNSPSC Code: 12352200
Volume: 200 µL

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AdvanceBio Sialidase V, Recombinant is a highly purified recombinant enzyme specifically designed for the selective removal of terminal sialic acid residues from glycoproteins, oligosaccharides, and complex glycans. Functioning efficiently under mild conditions, it enables accurate profiling and structural analysis of glycan compositions in biopharmaceutical research and quality control. Its broad substrate specificity allows effective desialylation of various linkages, including alpha2-3, alpha2-6, and alpha2-8 sialic acids, supporting detailed glycan mapping and characterization workflows.

This enzyme is ideal for applications in monoclonal antibody characterization, biosimilar development, and glycoproteome studies, providing critical insights into glycosylation patterns impacting efficacy and safety. Compatible with a wide range of Agilent analytical platforms, including the InfinityLab LC Series and AdvanceBio LC columns, it integrates seamlessly into established sample preparation and analysis pipelines. This reagent is recommended for use in combination with other AdvanceBio glycan sample prep kits, enzymatic tools, and chemistries to streamline comprehensive glycan analysis. Rigorous quality control ensures consistent performance and batch-to-batch reliability, making it a trusted choice for researchers focused on advancing glycoprotein characterization in both discovery and regulated environments.