Agilent, GK80080, AdvanceBio β(1-4)-Galactosidase, Recombinant

AdvanceBio β(1-4)-Galactosidase, Recombinant

Specifications:
Concentration: 2 U/mL
Enzyme Applications: Glycan analysis, Glycan digestion
Enzyme Formulation: Sterile-filtered solution in 20 mM Tris HCl, 25 mM NaCl, pH 7.5
Enzyme Source: Recombinant, expressed in E. coli, from Streptococcus pneumoniae
Enzyme Specificity: Cleaves nonreducing terminal β(1-4)-linked galactoseresidues from complex carbohydrates and glycoproteins
Enzyme Unit Definition: One unit of AdvanceBio β(1-4)-Galactosidase is defined as theamount of enzyme required to catalyze the release of 1 μmolof o-nitrophenyl (oNP) from o-nitrophenyl-β-galactopyranosideper minute, at pH 6.0 and 37 °C.
Storage Temp Conditions: Refrigeration required, 2-8°C
UNSPSC Code: 12352200
Volume: 100 µL

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AdvanceBio β(1-4)-Galactosidase, Recombinant is an enzymatic reagent designed to specifically cleave terminal β(1-4) linked galactose residues from glycoproteins and complex carbohydrates. This high-purity recombinant enzyme ensures consistent performance in the detailed characterization of glycan structures, making it ideal for biopharmaceutical analysis and academic research. Researchers utilize this enzyme to simplify complex glycan profiles, supporting in-depth structural elucidation of therapeutic antibodies, fusion proteins, and other biologics.

The reagent is supplied in a convenient format for seamless integration into routine workflows. It efficiently releases galactose residues under mild reaction conditions, preserving the integrity of sensitive biomolecules. This enzyme is fully compatible with a range of Agilent sample preparation tools and systems, including AdvanceBio Glycan Mapping columns and 1260 Infinity II LC systems.

Common applications include glycosylation profiling, quality control in pharmaceutical development, and biomarker discovery. The consistent lot-to-lot activity supports reliable results for both high-throughput and detailed analytical studies. The product can be used alongside other glycosidases for sequential or combinatorial digestion strategies, facilitating comprehensive glycoprotein analysis workflows. AdvanceBio β(1-4)-Galactosidase, Recombinant is an essential solution for laboratories seeking precise, reproducible removal of β(1-4)-linked galactose residues.