Agilent, GK80110, Enzymatic deglycosylation kit for N-linked and simple O-linked glycans

Enzymatic deglycosylation kit for N-linked and simple O-linked glycans. Contains all enzymes and reagents needed to remove all N-linked glycans and simple O-linked glycans (including polysialylated) from glycoproteins. See the product manual for an example using bovine fetuin.

N-glycanase (PNGase F) cleaves all N-linked complex, hybrid or high mannose oligosaccharides unless α(1,3) core fucosylated (asparagine must be peptide-bonded at both termini).

There is no such universal endoglycosidase for O-glycans. These have to be trimmed down to Gal-GalNAc-O-S/T before O-glycanase (GK80090) can remove the Gal-GalNAc structure. Modification of this core with, e.g., sialic acid will block O-glycanase.

Sialidase A (GK80040) is included in the kit and can remove terminal sialic acid from sialylated T antigen/Core 1 (Sia-Gal-GalNAc-O-S/T) to reveal core 1 Gal-GalNAc. The GK80115 extender kit (β(1,4)Galactosidase and β-N-Acetylglucosaminidase), available separately, can assist with removal of e.g. core 2 structures (GlcNAc-Gal-GalNAc-S/T). For more extended and complex O-glycans structures these enzymes will not be sufficient to reveal Gal-GalNAc. For more on O-GalNAc glycan core structures, please see references Specifications: Application: Epitope and binding site analysis Enzyme Applications: Control glycoprotein providedDeglycosylates up to 2 mg of glycoproteinNative and denaturing protocolsNo degradation of proteinRemoves O-linked sugars containing polysialic acid **********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.********** The Enzymatic Deglycosylation Kit enables efficient and reliable removal of N-linked and simple O-linked glycans from glycoproteins, streamlining sample preparation for advanced glycan analysis. Designed for both manual and automated workflows, this kit provides the essential enzymes and optimized buffers required for complete and reproducible deglycosylation while preserving the integrity of the protein backbone. Suitable for a broad range of biological samples, it is ideal for applications such as glycoprotein characterization, biotherapeutic development, antibody quality control, and biomarker discovery. Researchers benefit from rapid protocol times and straightforward procedures, enabling downstream analysis by liquid chromatography, capillary electrophoresis, and mass spectrometry. The kit is compatible with Agilent’s portfolio of LC and CE instruments, including the 1290 Infinity LC and 7100 CE systems, as well as with platforms from other major analytical equipment providers. Used in conjunction with Agilent glycan labeling and purification kits, it forms a comprehensive solution for in-depth glycan profiling. This product serves molecular biology, pharmaceutical, and clinical research laboratories seeking robust and reproducible deglycosylation for structural analysis and quantification of glycosylation patterns in complex proteins.