Agilent, GKE-5006A, AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

Specifications:
Application: Glycoprotein deglycosylation
Concentration: ≥2.5 U/mL
Enzyme Applications: Preparation of deglycosylated proteins for molecular weight estimation or crystallography studiesRelease of intact N-linked glycans from glycopeptides and glycoproteinsStructure-function studies of N-glycosylated glycoproteins
Enzyme Formulation: 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known asPNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 daltons
Unit: 100 mU
Volume: 40 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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AdvanceBio N-Glycanase is a highly purified recombinant PNGase F enzyme solution with an activity of at least 2.5 units per milliliter, designed for efficient and specific release of N-linked glycans from glycoproteins. This reagent is optimized for workflows in biopharmaceutical and glycoprotein characterization, enabling comprehensive analysis of glycosylation patterns that are critical for therapeutic antibody development, quality control, and biomarker discovery.

Suitable for both denatured and native glycoprotein substrates, the enzyme ensures complete deglycosylation in sample preparation protocols for HPLC, UHPLC, and mass spectrometry applications. AdvanceBio N-Glycanase delivers rapid and reliable glycan release, making it compatible with Agilent’s AdvanceBio Glycan Mapping columns, LC systems, and glycan labeling kits. It is also suitable for use with other major analytical platforms and workflows involving glycan profiling, ensuring broad utility in research and regulated laboratory settings.

The product is supplied in a convenient liquid format to facilitate ease of use and reproducibility. Ideal for research, development, and manufacturing environments, it supports advanced studies in structural biology, bioprocess monitoring, and regulatory compliance for biologics characterization.