Agilent, GKE-5006B, AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn.  Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

Specifications:
Concentration: ≥2.5 U/mL
Enzyme Applications: Preparation of deglycosylated proteins for molecular weight estimation or crystallography studiesRelease of intact N-linked glycans from glycopeptides and glycoproteinsStructure and function studies of N-glycosylated glycoproteins
Enzyme Formulation: 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 daltons
Unit: 200 mU
Volume: 80 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
AdvanceBio N-Glycanase is an optimized recombinant PNGase F enzyme solution with an activity of at least 2.5 units per milliliter. It efficiently cleaves N-linked oligosaccharides from glycoproteins, enabling detailed characterization of glycan structures in biopharmaceutical and academic research. This enzyme is highly effective for deglycosylation prior to HPLC, UPLC, LC-MS, or CE analysis, supporting workflows in monoclonal antibody characterization, quality control, biosimilar comparability, and glycosylation profiling in complex protein samples. The product delivers rapid deglycosylation under both denaturing and native conditions, minimizing sample preparation time and ensuring reproducibility. Its formulation is fully compatible with Agilent’s AdvanceBio Glycan Mapping columns and glycan labeling kits, as well as a variety of analytical platforms for glycan analysis. Researchers benefit from reliable batch-to-batch consistency and performance in high-throughput environments. The enzyme is ideal for laboratories focused on biotherapeutic development, cell line engineering, and glycoscience studies that demand sensitive and specific detection of N-glycans. AdvanceBio N-Glycanase is supplied as a ready-to-use solution, facilitating seamless integration into established sample preparation and analytical workflows for comprehensive glycoprotein and glycan characterization.