Agilent, GKE-5008, AdvanceBio Endo F2, recombinant

AdvanceBio Endo F2, recombinant. Enzyme cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Includes 5X reaction buffer, which when diluted gives 200 mM sodium acetate pH 4.5.

Specifications:
Enzyme Applications: Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins.
Enzyme Formulation: 10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase.
Enzyme Specificity: The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide, or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.
Enzyme Unit Definition: One milliunit is defined as the amount of enzyme that will hydrolyze 1 nM of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C.
Unit: 6 mU
Volume: 60 µL
pH Optimum: 4.5

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AdvanceBio Endo F2 is a highly purified recombinant endoglycosidase designed for efficient removal of N-linked glycans from glycoproteins. Engineered for optimal activity and minimal protease contamination, this enzyme specifically cleaves within the chitobiose core of high mannose and certain hybrid N-glycans, enabling researchers to study intact glycoprotein backbones and analyze glycan structures with confidence. Its high specificity and recombinant production provide consistent performance and batch-to-batch reliability, making it ideal for workflows in glycoprotein characterization, biopharmaceutical quality control, and structural biology studies. AdvanceBio Endo F2 is compatible with Agilent high-performance liquid chromatography HPLC systems and mass spectrometry MS platforms, and integrates seamlessly with Agilent AdvanceBio columns and enzymatic sample preparation kits. Applications include glycan profiling, confirmation of glycan occupancy, and analysis of recombinant protein therapeutics. The enzyme is supplied with detailed protocols to support use in regulated laboratory environments. Its streamlined workflow reduces sample preparation time and maintains high sample integrity, contributing to accurate glycosylation analysis in discovery, development, and quality assurance settings. This reagent is an essential component for advanced biopharmaceutical and glycomics research applications requiring precise and efficient deglycosylation.