Agilent, GKE-5010D, AdvanceBio N-Glycanase-plus (PNGase F), ≥10 U/mL

AdvanceBio N-Glycanase-plus (PNGase F), ≥10 U/mL. Enzyme releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. A recombinant form of PNGase F, 200mU, 1mMEDTA. GKE-5010D pack size does not ship with reaction buffers, however reaction buffers are available on request.

Specifications:
Concentration: 10 U/mL
Enzyme Applications: To obtain efficient deglycosylation of glycoprotein substrates under nondenaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation: 20 mM Tris HCl pH 7.5, containing 1 mM EDTA and 50 mM NaCl
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at pH 7.5 and 37°C.
Molecular Weight: ~35,000 daltons
Unit: 1 U
Volume: 100 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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AdvanceBio N-Glycanase-plus is a high-performance recombinant PNGase F enzyme supplied at a concentration of at least 10 units per milliliter. This product efficiently removes N-linked glycans from glycoproteins, making it an essential tool for researchers performing glycan profiling, characterization, and structural analysis. It is optimal for analytical workflows in biopharmaceutical development, quality control, and glycomics studies, ensuring reliable and consistent deglycosylation across various sample types including monoclonal antibodies, recombinant proteins, and plasma-derived products.

Designed for compatibility with Agilent’s AdvanceBio Glycan Mapping solutions, this enzyme integrates seamlessly into established HPLC and LCMS glycan analysis workflows. It complements products such as AdvanceBio Glycan Mapping columns and labeling kits, enhancing accuracy and throughput when combined with Agilent’s 1290 Infinity LC, 1260 Infinity II LC, and UHPLC systems. The streamlined protocol supports both manual and automated sample preparation, minimizing downtime while maximizing reproducibility. Purified to high standards, the enzyme provides low background activity and minimal protease contamination, delivering precise results even in complex biological samples. AdvanceBio N-Glycanase-plus is ideally suited for laboratories seeking robust deglycosylation performance for comprehensive glycosylation analysis and regulated biotherapeutic characterization.