Agilent, GKE-5011A, AdvanceBio PNGase A (almond), 2 mU

AdvanceBio PNGase A (almond), 2 mU. Enzyme cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in insects and plants. Incudes reaction buffer: 50 mM citrate-phosphate buffer, pH 5.0, 0.02% sodium azide.

Specifications:
Enzyme Formulation: Lyophilized powder containing citrate-phosphate buffer, pH 5.0.
Enzyme Source: Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase.
Enzyme Specific Activity: > 5 units/mg
Enzyme Specificity: PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.
Molecular Weight: 54.2 and 21.2 kDa
Unit: 2 mU
pH Optimum: 5
pH Range: 4-6

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AdvanceBio PNGase A (almond), 2 mU is a high-purity glycosidase enzyme specifically designed for efficient release of N-linked glycans from glycoproteins. Isolated from almond sources, this enzyme exhibits broad substrate specificity, making it suitable for deglycosylation of glycoproteins derived from non-mammalian origins, such as plants, fungi, and insects, where PNGase F shows limited activity. With its precise enzymatic activity, it facilitates robust and consistent glycan analysis workflows. This product is ideal for applications in biopharmaceutical research, quality control, and structural characterization studies focused on glycoprotein therapeutics and biosimilars. It is optimized for compatibility with Agilent AdvanceBio Glycan Mapping columns and seamlessly integrates with LC and LC/MS systems, including Agilent 1290 Infinity II and 1260 Infinity II LC platforms. The enzyme is supplied in a user-friendly format, supporting both manual and automated sample preparation protocols. Researchers can achieve accurate, reproducible results for comprehensive glycan profiling, aiding in critical decision-making during product development and regulatory submission. AdvanceBio PNGase A complements Agilent’s glycan analysis kits and sample preparation tools, supporting the end-to-end characterization and monitoring of glycosylation in complex biological samples.