Agilent, GKE-5011B, AdvanceBio PNGase A (almond), 10 mU

AdvanceBio PNGase A (almond), 10 mU. Enzyme cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in insects and plants. Includes reaction buffer: 50 mM citrate-phosphate buffer, pH 5.0, 0.02% sodium azide.

Specifications:
Enzyme Formulation: Lyophilized powder containing citrate-phosphate buffer, pH 5.0.
Enzyme Source: Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase.
Enzyme Specific Activity: > 5 units/mg
Enzyme Specificity: PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.
Molecular Weight: 54.2 and 21.2 kDa
Unit: 10 mU
pH Optimum: 5
pH Range: 4-6

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AdvanceBio PNGase A (almond), 10 mU, is a high-purity glycosidase enzyme designed for precise N-glycan release from glycoproteins, including those with core alpha-1,3-fucosylation typically found in plant or insect-derived samples. Sourced from almond, this enzyme facilitates efficient and gentle deglycosylation, particularly suited for workflows where PNGase F is ineffective. It is optimized for use in analytical applications such as characterization of recombinant biotherapeutics, glycan structural analysis, and glycoprotein profiling. The product is supplied in an activity unit format, allowing for controlled application in both manual and automated sample preparation protocols. Compatibility with Agilent AdvanceBio Glycan Mapping workflows ensures seamless integration with liquid chromatography and mass spectrometry systems, including Agilent 1290 Infinity II and 1260 Infinity II LC platforms. It can be used alongside AdvanceBio Glycan Mapping kits, labeling reagents, and glycan standards for comprehensive glycomics studies. This reagent is ideal for academic, biopharmaceutical, and quality control laboratories seeking high specificity and consistent performance in glycosylation analysis. Its robust performance ensures reliable results, supporting critical decision making in research and development programs focused on glycoprotein characterization.