Agilent, GKE-5016A-05, AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Supplied as a 5 x 100 mU vials of GKE-5016A. Includes5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications).

Specifications:
Concentration: 2.5 U/mL
Enzyme Formulation: 20 mM Tris-HCl, 50 mM NaCl, (pH 7.5)
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 Daltons
Unit: 500 mU
Volume: 40 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
AdvanceBio N-Glycanase EDTA free is a highly purified recombinant Peptide N Glycosidase F enzyme supplied at a concentration of at least 2.5 Units per milliliter. This product is specifically designed for rapid and efficient enzymatic release of N linked oligosaccharides from glycoproteins, making it ideal for glycan profiling and structural analysis in biopharmaceutical research and quality control. Its EDTA free formulation ensures compatibility with a broad range of sample buffers and maintains the integrity of metal ion dependent target proteins and enzymatic assays. The enzyme delivers consistent and reliable performance for both denatured and native substrates and is suitable for use in various sample preparation workflows.

Typical usage scenarios include glycoprotein characterization, biosimilar comparability studies, and release testing during process development. Applications extend across HPLC, LC MS, and CE based glycan analysis workflows. The enzyme integrates seamlessly with Agilent AdvanceBio Glycan Mapping columns, reagents, and sample preparation kits for streamlined, reproducible results. Additionally, it is compatible with automated platforms such as the Agilent AssayMAP and Bravo systems, supporting high throughput glycan analysis. AdvanceBio N-Glycanase EDTA free provides researchers with a robust and flexible solution for the detailed study of protein glycosylation patterns.