Agilent, GKE-5016B-1, AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Supplied as 5 x 200 mU vials of GKE-5016B. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications).

Specifications:
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Unit: 1000 mU

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AdvanceBio N-Glycanase is a highly purified, EDTA-free recombinant peptide N-glycosidase F supplied at a concentration of at least 2.5 units per milliliter. Designed for precise and efficient enzymatic cleavage, it liberates N-linked oligosaccharides from glycoproteins without metal chelator interference, ensuring accurate downstream analysis. This enzyme optimally supports workflows in biopharmaceutical research, including glycan profiling, structural analysis, and quality control of monoclonal antibodies and therapeutic proteins. It is particularly suited for sample preparation ahead of mass spectrometry, HPLC, and UPLC applications. The formulation guarantees compatibility with a range of analytical systems such as Agilent’s 1260 Infinity II and 1290 Infinity II LC platforms. Ideal for use with AdvanceBio Glycan Mapping columns and associated sample prep kits, it ensures reproducible and reliable results. The absence of EDTA makes it compatible with a broader array of reaction buffers and sensitive assays that could otherwise be compromised by chelators. AdvanceBio N-Glycanase delivers the high specificity and activity required for rigorous glycan analysis in both research and regulated environments. This reagent streamlines complex sample preparation steps, enabling robust glycoprotein de-glycosylation for comprehensive characterization.