Agilent, GKE-5016D, AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL . A recombinant form of PNGase F (1U, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Does not include reaction buffers that ship with "A" (100 mU) and "B" (200 mU) pack sizes. Please contact us if you require buffers.

Specifications:
Concentration: 2.5 U/mL
Enzyme Formulation: 20 mM Tris-HCl, 50 mM NaCl (pH 7.5)
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 Daltons
Unit: 1 U
Volume: 400 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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Agilent AdvanceBio N-Glycanase (PNGase F, EDTA free) is a high-purity enzyme designed for the efficient and specific release of N-linked oligosaccharides from glycoproteins, even in complex sample backgrounds. With an activity of at least 2.5 units per milliliter, this reagent is optimized for use in both research and quality control environments, ensuring reliable and reproducible deglycosylation. The EDTA-free formulation is compatible with applications where the preservation of metal cofactors is essential, such as structural or functional analysis of metalloproteins.

This N-Glycanase is widely used in glycosylation analysis workflows, biopharmaceutical characterization, and glycan profiling studies. It is ideal for sample preparation prior to subsequent LC, LC-MS, or capillary electrophoresis, seamlessly integrating with Agilent AdvanceBio Glycan Mapping columns, Agilent 1260 and 1290 Infinity LC Systems, and Agilent high-resolution mass spectrometers. The enzyme is suitable for automated platforms or manual workflows, supporting rigorous analytical requirements for monoclonal antibody development and other biotherapeutic applications. AdvanceBio N-Glycanase delivers consistent, high-quality results, making it a vital component in glycomics research and detailed glycan structure elucidation.