Agilent, GKE-5020D, AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL. A recombinant form of PNGase F (1U, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. GKE-5020D pack size does not ship with reaction buffers, although reaction buffers are available on request.

Specifications:
Concentration: ≥10 U/mL
Enzyme Applications: To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation: 20 mM Tris HCl pH 7.5, 50 mM NaCl
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 Daltons
Unit: 1 U
Volume: 100 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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AdvanceBio N-Glycanase-ultra is a high-purity recombinant Peptide N-Glycosidase F formulated without EDTA, offering activity levels of at least 10 units per milliliter. This enzyme rapidly cleaves N-linked oligosaccharides from glycoproteins, enabling comprehensive glycan analysis crucial for biopharmaceutical development, quality control, and characterization. Its EDTA-free composition makes it fully compatible with downstream processes sensitive to metal chelators, such as mass spectrometry and HPLC-based workflows.

Optimized for reliable performance, this enzyme supports rigorous applications including release and profiling of N-glycans from monoclonal antibodies, fusion proteins, and other therapeutics. The preparation ensures efficient deglycosylation under both denaturing and native conditions, reducing sample preparation time while preserving protein integrity.

AdvanceBio N-Glycanase-ultra is suitable for use with Agilent glycan analysis solutions, such as AdvanceBio Glycan Mapping columns and LC-MS systems, but is also compatible with a broad range of analytical instrumentation for glycomic and proteomic studies. It is an indispensable reagent for researchers and analytical laboratories focused on glycoprotein structure-function studies, ensuring accurate and reproducible glycan characterization within regulatory or discovery settings.