Agilent, GKE-5020D-10, AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL. Enzyme (a recombinant form of PNGase F, EDTA free) releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Supplied as 10 vials of GKE-5020D (10 x 1 U, ≥10 U/ml). GKE-5020D pack size does not ship with reaction buffers, although reaction buffers are available on request.

Specifications:
Concentration: ≥10 U/mL
Enzyme Applications: To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation: 20 mM Tris HCl pH 7.5, 50 mM NaCl
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 Daltons
Unit: 10 U
Volume: 100 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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AdvanceBio N-Glycanase-ultra is an optimized recombinant PNGase F enzyme solution, offered at a concentration of at least 10 units per milliliter and formulated without EDTA. Designed for high-efficiency release of N-linked glycans from glycoproteins, this reagent is ideally suited for biopharmaceutical development, glycoprotein characterization, and quality control processes requiring precise glycan analysis. Its EDTA-free formulation ensures compatibility with metal-sensitive samples and downstream mass spectrometry or HPLC workflows.

This enzyme performs rapid and complete deglycosylation under standard denaturing or native conditions, increasing throughput and confidence in glycan profiling. Typical applications include therapeutic antibody glycan profiling, structural elucidation of protein glycosylation, and bioprocess characterization.

AdvanceBio N-Glycanase-ultra integrates seamlessly with Agilent AdvanceBio Glycan Mapping workflows and complements Agilent HPLC and LC-MS instruments, including 1290 Infinity LC and 6545 Q-TOF MS systems. It is also suitable for use with AdvanceBio Glycan Mapping columns and kits, ensuring streamlined sample preparation and reliable results. The product is quality controlled for high specificity and activity, making it an essential reagent for laboratories engaged in biologics development and comprehensive glycoprotein analytics.