Agilent, GKIB-001, AdvanceBio InstantAB Human α-1-acid glycoprotein N-glycan Library

AdvanceBio InstantAB Human α-1-acid glycoprotein N-glycan Library. Represents a total pool of N-linked glycans released from human α1-acid glycoprotein and labeled with InstantAB, constituting a heterogenous mixture of core non-fucosylated bi-, tri-, and tetraantennary glycans with various degrees of sialylation (NeuAc) and some with outer arm fucose residues and lactosamine repeats, consistent with N-glycans previously reported for human α1-acid glycoprotein. The biantennary glycans can have one or two sialic acid residues. The triantennary and tetraantennary glycans can have from one to four sialic acid residues and may be substituted with fucose that result in formation of sialyl Lewis X-like structure.
The sialic acid residues are found both in α(2,3) and α(2,6) linkages. The composition of the library is approximately as follows: monosialylated glycans ~1%, di-sialylated ~20%, tri-sialylated ~51% and tetrasialylated ~27%. Triantennary glycans are a major component and constitute ~44% of total glycans present in the Human α1-acid glycoprotein N-linked Glycan Library. The reducing termini are derivatized with the fluorescent dye, InstantAB. Specifications: Label: InstantAB Unit: 70 pmol **********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.********** The AdvanceBio InstantAB Human α-1-acid glycoprotein N-glycan Library provides a high-quality, ready-to-use reference standard for N-glycan profiling and characterization in biopharmaceutical research. This library contains a well-characterized mixture of N-glycans released from human α-1-acid glycoprotein, labeled with InstantAB dye to ensure sensitive fluorescence detection. The product is designed to support glycan analysis workflows such as method development, system suitability testing, or assay calibration, providing researchers with confidence in their HILIC-UHPLC and LC-MS glycan assays. Ideal for laboratories focused on biotherapeutic development, glycoengineering, biosimilar comparability, and biomarker discovery, this glycan library enables reliable identification and quantitation of N-glycan structures. It is fully compatible with leading Agilent AdvanceBio Glycan Mapping columns, the Agilent 1290 Infinity II LC system, and mass spectrometry platforms, supporting seamless integration into existing analytical workflows. The library is suitable for routine quality control, glycan structure validation, and as a positive control in glycan release and labeling efficiency studies. Its comprehensive documentation and rigorous QC testing help ensure robust and reproducible results, making it an essential reference tool for glycan analysis and biopharmaceutical research.