Agilent, GKX-5003, β-N-Acetylhexosaminidase (jack bean)

β-N-Acetylhexosaminidase (jack bean). Enzyme releases nonreducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Includes 5x Incubation Buffer [500 mM sodium citrate phosphate (pH 5.0)] 

Specifications:
Concentration: 50 U/mL
Enzyme Applications: Useful for the structural analysis of isolated glycans and glycoconjugates. pH optimum/range: For glucosaminide 5.0 - 6.0, For galactosaminide 3.5 - 4.0
Enzyme Formulation: 20mM sodium citrate phosphate (pH 6.0)
Enzyme Source: Jack bean.
Enzyme Specificity: The enzyme exhibits a broad specificity, cleaving nonreducing terminal ß(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. This enzyme is useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with ß N-acetylhexosaminidase from S. pneumoniae (GK80050). We also offer this enzyme at a higher concentration - see GKX-5023. Higher enzyme concentrations may be necessary to remove bisecting ß(1-4)-linked GlcNAc residues in complex N-glycans.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP N-acetyl-ß-D-glucosaminide per minute at pH 5.0 and 37°C.
Molecular Weight: 100 - 110 kDa
Unit: 5 U
Volume: 100 µL

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Derived from jack bean, this highly purified enzyme preparation efficiently catalyzes the hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. It is ideal for glycoprotein structural analysis, oligosaccharide sequencing, and glycan profiling studies in biochemical and glycobiology research. The enzyme displays broad substrate specificity, enabling comprehensive digestions required for characterizing complex glycan structures in biological samples.

This reagent is an essential tool for applications such as monitoring glycoprotein processing, investigating lysosomal storage disorders, and supporting quality control in biopharmaceutical development. Consistent performance and robust activity make this product suitable for use in both manual and automated workflows.

The enzyme is compatible with a variety of analytical platforms, including high-performance liquid chromatography HPLC, mass spectrometry MS, and capillary electrophoresis CE, facilitating downstream detection and quantification of released monosaccharides and oligosaccharides. It can be paired with other Agilent glycan analysis reagents and sample preparation kits to achieve reliable and reproducible results. Each lot is subjected to stringent quality control to ensure activity and purity, providing dependable performance for critical research and development applications.