Agilent, GKX-5010-50, α(1-2,3,6)-Mannosidase

α(1-2,3,6)-Mannosidase. Mannosidase (jack bean) 50 U, ≥150 U/ml, releases nonreducing terminal α(1-2,3,6)-linked mannose from oligosaccharides. Supplied as 5 vials of GKX-5010. Includes incubation buffer [(500 mM sodium acetate, 10 mM zinc chloride (pH 5.0)].

Specifications:
Concentration: 150 U/mL
Enzyme Formulation: 20 mM Tris-HCl, 20 mM NaCl (pH 7.5)
Enzyme Source: Jack bean.
Enzyme Specificity: The enzyme has broad specificity, cleaving a(1-2,3,6)-linked mannose, although some kinetic preference has been observed (a1-2, 6 > 3). The enzyme will not cleave a single a(1-6) linked mannose residue from core ß-mannose, but will, however, remove a single a1-3 linked mannose from the core ß-mannose. By using enzyme concentrations of around 50 U/ml and extended incubation times (up to 18 hours) at 37°C, complete removal of all nonreducing terminal a-linked mannose residues may be achieved. To expedite glycan sequencing studies, the sluggish activity of GKX-5010 jack bean a mannosidase toward a(1-6)-linked mannose residues can be overcome by using the enzyme in combination with the a mannosidase from Xanthomonas mannihotis that rapidly cleaves a(1-6) linkages. The X. mannihotis a-mannosidase may inhibit the action of other mannosidases if a branched (a1-6) mannose is present in the substrate, so is typically added after incubation of the substrate with jack bean a-mannosidase (GKX-5010).
Enzyme Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze 1 µmole of pNP-α-mannopyranoside per minute at pH 4.5 and 37°C.
Molecular Weight: ~190 kD
Unit: 50 U
Volume: 70 µL
pH Optimum: 4.5

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This highly purified glycosidase specifically catalyzes the hydrolysis of non-reducing terminal alpha-1,2, alpha-1,3, and alpha-1,6-linked mannose residues from N-linked oligosaccharides. Its broad substrate specificity makes it essential for detailed structural characterization of complex glycoproteins and oligosaccharides. The enzyme is optimized for in vitro glycan analysis workflows, facilitating the sequential or selective removal of mannose residues during glycan mapping, glycoengineering, or epitope analysis in biopharmaceutical development.

Supplied as a ready-to-use solution, this product shows consistent, high activity across a variety of biological and recombinant glycoproteins, including antibodies and therapeutic enzymes. It is compatible with Agilent’s range of glycan labeling kits, HPLC, and LC/MS instruments, as well as with other common sample preparation and analysis systems. Researchers can use it for quality control, structural elucidation, and monitoring glycan heterogeneity in research, preclinical, and regulated environments.

This enzyme complements Agilent’s comprehensive glycan analysis solutions, enabling robust and reliable workflows. It is ideal for academic, clinical, and industrial laboratories focused on glycoprotein research, therapeutic antibody production, or biomarker discovery. Product includes detailed protocol for optimal performance and reproducibility.