Agilent, GKX-5023, β-(1-2,3,4,6)-Hexosaminidase, sequencing-grade (jack bean)

β-(1-2,3,4,6)-Hexosaminidase, sequencing-grade (jack bean). Enzyme releases nonreducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Supplied at a higher concentration than GKX-5003. Higher enzyme concentrations may be necessary to remove bisecting β(1-4)-linked GlcNAc residues in complex N-glycans. Includes 5x Enzyme reaction buffer (500 mM sodium phosphate, pH 5.5).

Specifications:
Concentration: 300 U/mL
Enzyme Applications: The enzyme exhibits a broad specificity, cleaving non-reducing terminal ß(1-2,3,4, and 6)-linked-N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. It has proved very useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with ß-N-Acetylhexosaminidase from S. Pneumoniae (GK80050).
Enzyme Formulation: 20mM sodium citrate phosphate (pH 6.0)
Enzyme Source: Jack bean.
Enzyme Unit Definition: One unit of ß-N-Acetylhexosaminidase is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from pNP-N-acetyl-ß-D-glucosaminide per minute at pH 5.0 and 37°C.
Molecular Weight: ~67 kD (from literature)
Unit: 15 U
Volume: 50 µL
pH Range: 5.5-6

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Agilent's sequencing-grade β-(1-2,3,4,6)-Hexosaminidase, sourced from jack bean, enables highly specific and efficient cleavage of terminal N-acetylglucosamine (GlcNAc) residues from complex glycan structures. This enzyme is optimized for glycoprotein structural analysis, glycan sequencing, and biomarker discovery in proteomics and glycomics research workflows. It ensures precise removal of β-linked GlcNAc units at the 1-2, 1-3, 1-4, and 1-6 positions, facilitating detailed characterization of N- and O-linked glycans.

The sequencing-grade purity maximizes compatibility with downstream mass spectrometry and HPLC analyses by minimizing contaminating protease or glycosidase activity. Recommended applications include mapping of glycosylation sites, elucidation of glycan branching patterns, and validation of biotherapeutics during process development.

The enzyme integrates seamlessly with Agilent’s AdvanceBio Glycan Mapping kits, LC columns, and high-resolution Q-TOF and triple quadrupole mass spectrometry systems, providing comprehensive support for complex glycan analysis. Its proven performance improves reproducibility and sensitivity in quantitative and qualitative studies, making it a valuable tool for academic, clinical, and industrial research settings where the structural elucidation of glycoproteins is critical.