Agilent, K007811-8, QIFIKIT®, Series of coated beads, Flow Cytometry, 10 calibrations

QIFIKIT®, Series of coated beads, Flow Cytometry, 10 calibrations. Ready-to-use kit

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This kit contains a series of precisely calibrated microparticles designed for quantifying cell surface antigens by flow cytometry. Each bead population is pre-coated with defined amounts of mouse monoclonal antibodies, enabling reliable construction of standard curves and absolute quantification of antigen density on cells. The kit provides ten distinct calibration points, supporting enhanced accuracy across a broad dynamic range.

Researchers use this system for standardized quantitative flow cytometric analyses in immunology, oncology, and cell biology, particularly to assess receptor or biomarker expression and validate antibody binding. Typical applications include characterization of lymphocyte populations, assessment of therapeutic antibody binding sites, and standardization of laboratory protocols across instruments or laboratories.

Compatible with most major flow cytometers, such as BD FACSCanto, BD LSRFortessa, and Agilent NovoCyte, these coated beads integrate seamlessly into multicolor immunophenotyping workflows. This product is often used alongside fluorochrome-conjugated antibodies and related calibration reagents for comprehensive cell surface analysis. By providing traceable quantification, the kit facilitates data comparison and reproducibility in both basic research and clinical studies. The included detailed protocol ensures straightforward setup and consistent results for routine or high-throughput laboratory settings.