Agilent, K231111-5, IntraStain

IntraStain. Two-step fixation and permeabilization, Flow Cytometry, 100 tests. Includes: Reagent A 10 mL, Reagent B 10 mL. Ready-to-use.

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IntraStain is a high-quality reagent formulated for the reliable intracellular staining of proteins, cytokines, and other cellular markers in flow cytometry and immunofluorescence applications. Designed for efficient permeabilization and fixation, this product ensures optimal access of antibodies to intracellular targets while preserving cell morphology and antigenicity. Its robust performance allows for precise detection and quantification of intracellular molecules, making it ideal for studies involving immune cell phenotyping, signal transduction research, and functional assays such as cytokine profiling or phosphoprotein analysis.

Common usage scenarios include multiparametric flow cytometry assays in immunology, cancer biology, and developmental biology. It is especially well-suited for protocols analyzing cell signaling events, intracellular protein expression, or performing combined surface and intracellular marker detection. The reagent is fully compatible with a broad range of flow cytometers, including Agilent NovoCyte cytometers, and can be seamlessly integrated into existing staining workflows. For maximum efficiency, it pairs effectively with fluorochrome-conjugated antibody panels and is suitable for use alongside Agilent antibody reagents and buffers. Comprehensive technical support and detailed protocols are available to facilitate implementation in research laboratories seeking reliable and reproducible intracellular staining solutions.