Agilent, K231111-8J, IntraStain, Fixation and Permeabilization Kit for Flow Cytometry, JP

IntraStain, Fixation and Permeabilization Kit for Flow Cytometry, JP

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This fixation and permeabilization kit is optimized for efficient intracellular staining in flow cytometry applications, supporting robust and reproducible detection of cytoplasmic and nuclear antigens. Designed to deliver high cell viability and minimal background, the kit ensures preservation of cellular morphology during fixation and facilitates optimal antibody access with its permeabilization reagents. This kit is suitable for multiparameter flow cytometry analysis of immune cell subsets, signal transduction studies, and assessment of intracellular cytokines, transcription factors, or phosphorylated proteins. The reagent formulation is compatible with a wide array of fluorochrome-conjugated antibodies and staining panels, making it applicable for use with most major flow cytometers, including Agilent NOVA, BD FACSCanto, and similar systems. As an essential tool for immunology, cancer research, and cell signaling investigations, this solution streamlines complex protocols by providing ready-to-use buffers and clear instructions, reducing hands-on preparation time. It seamlessly integrates with complementary flow cytometry reagents such as surface staining buffers and viability dyes, enhancing flexibility for customized assay design. Researchers can rely on consistent performance across experiments, enabling reproducible quantification of intracellular targets in whole blood, cell suspensions, or fixed sample types.