Agilent, PL1180-5301, 1 AdvanceBio SEC 300Å, 7.8 x 300 mm, 2.7 µm, HPLC column

1 AdvanceBio SEC 300Å, 7.8 x 300 mm, 2.7 µm, HPLC column. A size exclusion chromatography (SEC) column for efficient aggregate analysis of proteins, including monoclonal antibodies. The resolving range is 5-1,250 kDa.

Specifications:
Brand: AdvanceBio SEC
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)Proteins
Particle Size: 2.7 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: 0.02% Sodium Azide
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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This size exclusion chromatography (SEC) column is optimized for high-resolution analysis of biomolecules, specifically protein aggregates and monomers, with a pore size of 300 angstroms. Featuring a 7.8 x 300 mm dimension and 2.7 micron particle size, it offers enhanced efficiency and robust performance, delivering reproducible results for biopharmaceutical research and quality control laboratories. Its advanced silica-based design ensures minimal non-specific adsorption, making it ideal for the characterization of monoclonal antibodies, ADCs, and other complex proteins across a broad molecular weight range.

Engineered for compatibility with most standard HPLC and UHPLC systems, this column integrates seamlessly with Agilent 1100, 1200, 1260, and 1290 series, as well as other leading chromatography instruments. Typical usage scenarios include aggregate and fragment screening, purity assessment, and stability studies during process development. The column supports rapid throughput and excellent peak symmetry, making it suitable for regulated environments demanding secure and reliable biomolecule quantitation.

It is recommended for use in conjunction with Agilent AdvanceBio SEC guard columns and is compatible with standard aqueous SEC mobile phases. This makes the column a versatile and dependable choice for researchers analyzing the higher-order structure and size variants of therapeutic proteins and other macromolecules.