Agilent, WS0278-GP, N-glycanase, for GlykoPrep-plus, 700 μL

N-glycanase, for GlykoPrep-plus, 700 μL. Replacement enzyme for GlykoPrep-plus kits, 620 ul fill, ≥10 U/mL, EDTA-free

Specifications:
Application: Refill for GlykoPrep-plus glaycan preparatation kit
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic, acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.

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This high-quality N-glycanase enzyme solution is designed for efficient and specific release of N-linked glycans from glycoproteins. Supplied in a convenient 700 microliter format, it is optimized for use with GlykoPrep-plus workflows, ensuring streamlined glycan analysis and sample preparation. Researchers will benefit from its robust activity and high purity, which are essential for applications such as biotherapeutic characterization, glycomics profiling, quality control, and biomarker discovery.

Ideal for both routine and advanced laboratories, this enzyme is compatible with automated and manual protocols, supporting seamless integration into existing sample prep processes. Its performance is validated for use with Agilent’s GlykoPrep-plus kits and related glycan analysis systems, which include liquid chromatography and mass spectrometry platforms. By enabling the rapid and complete deglycosylation of a broad range of glycoprotein samples, this product helps ensure accurate, reproducible results in both research and regulated environments.

Pairing with complementary reagents and tools from the GlykoPrep-plus line maximizes throughput, precision, and workflow efficiency. The solution meets the rigorous standards required for reliable, high-sensitivity glycan analysis, making it an essential component in academic, pharmaceutical, and biotechnology research applications.