BD, 550257, BD Pharmingen™ PE Mouse Anti-Human CD73

Alternative Name: NT5E; 5' nucleotidase; 5'-NT; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Pre-B leukemia cell line
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 20 µl
Workshop Number: V B-CD73.3
RRID: AB_393561
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.