BD, 550274, BD Pharmingen™ Purified Rat Anti-Mouse CD31

Alternative Name: EndoCAM; GPIIA; PECA1; PECAM1; Platelet endothelial cell adhesion molecule
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a, κ
Immunogen: 129/Sv mouse-derived endothelioma cell line tEnd.1
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 15.625 µg/ml
RRID: AB_393571
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunocytochemistry: The MEC13.3 antibody is recommended to test for immunohistochemical staining of Zinc-fixed paraffin sections. Tissues tested were mouse spleen, lung, heart, and thymus. Immunohistochemistry of acetone-fixed frozen sections has been reported. The antibody stains endothelial cells on small and large blood vessels. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the MEC13.3 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination of biotinylated polyclonal anti-rat Igs (multiple adsorption) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). Alternatively, the anti-rat Ig HRP Detection Kit (Cat. No. 551013) can be used to accomplish the three-step staining procedure. The clone MEC13.3 is not recommended for formalin-fixed paraffin embedded sections
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.