BD, 550277, BD Pharmingen™ Purified Hamster Anti-Mouse CD3e

Alternative Name: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Reactivity: Mouse (QC Testing)
Isotype: Syrian Hamster IgG2, κ
Immunogen: Mouse T-cell receptor
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393573
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: For optimal indirect immunohistochemical staining, this antibody should be titrated (e.g suggested starting dilutions of 1:10 to 1:50) and visualized via a three step staining procedure in combination with a Biotin Mouse Anti-Hamster IgG2 antibody (Cat. No. 554029) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Investigators should note that this antibody is not recommended for paraffin-embedded sections.