BD, 550284, BD Pharmingen™ Purified Rat Anti-Mouse CD19

Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a, κ
Immunogen: Mouse CD19 Transfected Cell Line
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 15.625 µg/ml
RRID: AB_393579
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 1D3 antibody is recommended to test for immunohistochemical staining on acetone-fixed frozen sections. The clone 1D3 is not recommended for formalin-fixed paraffin embedded sections. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073).  For optimal indirect immunohistochemical staining, the 1D3 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-rat Ig (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB substrate (Cat. No. 550880). Alternatively, the anti-rat Ig detection kit may also be used (Cat. No. 551013). A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. An isotype control should be used at the same concentration as the antibody of interest. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.