BD, 550538, BD Pharmingen™ Purified Rat Anti-Mouse CD44

Alternative Name: Pgp-1; Ly-24; H-CAM; HERMES; ECMR-III; Hyaluronate Receptor
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b, κ
Immunogen: Dexamethasone-induced, SJL mouse spontaneous myeloid leukemia M1 cells myeloid leukemia M1
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
Concentration: 62.5 µg/ml
RRID: AB_393732
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The IM7 antibody specific for mouse CD44 is recommended to test for immunohistochemical staining of formalin-fixed paraffin and acetone-fixed frozen sections. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) is required for formalin-fixed paraffin sections. Tissues tested were mouse spleen and thymus. The antibody stains epithelial cell and T and b lymphocytes. The isotype control recommended for use with this antibody is purified rat IgG2b (Cat. No. 559478). For optimal indirect immunohistochemical staining, the IM7 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotin conjugated anti-rat IgG 2b (Cat. No. 550327) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.