BD, 550541, BD Pharmingen™ Purified Hamster Anti-Mouse CD61

Alternative Name: Itgb3; Integrin beta-3; INGRB3; Platelet glycoprotein IIIa; GP3A; GPIIIa
Reactivity: Mouse (QC Testing), Rat (Tested in Development)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Mouse T-cell Hybridoma 2B4 Vitronectin Receptor
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 250 µg/ml
RRID: AB_393735
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 2C9.G2 antibody specific for mouse CD61 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen and thymus. The antibody stains platelets activated T lymphocytes and polymorphonuclear granulocytes. The isotype control recommended for use with this antibody is purified hamster IgG (Cat. No. 550344). For optimal indirect immunohistochemical staining, the 2C9.G2 antibody should be titrated (1-10 to 1-50 dilution and visualized via a three-step staining procedure in combination with anti-hamster IgG cocktail (Cat. No. 550335) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources