BD, 551226, BD Pharmingen™ Purified Mouse Anti-Human MCP-1

Alternative Name: CCL2; C-C motif chemokine 2; Chemokine (C-C motif) ligand 2; MCAF; SCYA2
Reactivity: Human (QC Testing), Rhesus, Cynomolgus (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant Human MCP-1
Application: ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Neutralization (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_394103
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture: The purified 5D3-F7 antibody is useful as a capture antibody in a sandwich ELISA for measuring human MCP-1 protein levels. For specific methodology, please visit the protocols section of "ELISA and ELISPOT" on our web site, http://www.bdbiosciences.com/us/s/resources. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human MCP-1 in serum or plasma our Human MCP-1 BD OptEIA™ ELISA Set (Cat. No. 555179) or BD OptEIA ELISA Kit (Cat. No. 559107) are specially formulated and recommended. Neutralization: The NA/LE™ 5D3-F7 antibody (Cat. No. 554661) has been reported to be useful for neutralization of human MCP-1 bioactivity.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.