BD, 551850, BD Pharmingen™ PE Mouse Anti-Human CD126

Alternative Name: Interleukin 6 Receptor alpha chain; IL-6R alpha; IL-6Rα
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: CD126 Recombinant Protein
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 20 µl
Workshop Number: VI C63; IX 36
RRID: AB_394271
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE Mouse Anti-Human CD126 (Cat. No. 551850) can be used for the immunofluorescent staining (20 µg/10e6 cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface IL-6Ra.15 An appropriate purified immunoglobulin is PE Mouse IgG1, κ Isotype Control (Cat. No. 555749). ELISA: Purified Mouse Anti-Human CD126 (Cat. No. 551462) is useful as a capture for a sandwich ELISA that measures soluble human IL-6Ra protein levels. The M5 antibody can be paired with the M182 antibody and recombinant soluble human IL-6Ra as a standard.