BD, 553499, BD Pharmingen™ Purified Mouse Anti-Mouse IgG1[a]

Alternative Name: Igh-4a; Ighg1; IgG1
Isotype: Mouse SJL IgG2a, κ
Immunogen: Not reported
Application: ELISA (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394884
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Indirect ELISA: Purified mouse IgG1 (a allotype) [MN 557273] may be used as the antigen (e.g coating plates). Purified anti-mouse IgG1[a] allotype specific antibody [MN 553499] may be used at ~ 2 µg/ml followed by biotinylated anti-mouse IgG2a [b] allotype specific antibody [MN 553504] at ~ 2 µg/ml and avidin-HRP [MN 554058] for detection.