BD, 557906, BD Pharmingen™ Alexa Fluor® 647 Rat IgG2a, κ Isotype Control

Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse Pooled Immunoglobulin
Application: Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_396931
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). Important Note: This pre-titered antibody solution does not contain a cell permeabilization agent. It is necessary to include a cell permeabilization agent when using the pre-titered antibody solution to stain fixed and permeabilized cells with Cytofix/Cytoperm  Kit(Cat. No. 554714). BD Perm/Wash™ Buffer (Cat. No 554723) contains the permeabilization agent saponin and is useful for this purpose as described in the USAGE section below. USAGE for Intracellular Staining Resuspend 1 x 10e6 fixed and permeabilized cells in 5 µl of the pre-titered antibody solution and 45 µl of 1X Perm/Wash Buffer (Cat. No 554723). Incubate the cell suspension for 15 minutes (at RT or 4°C). Wash twice in 100 µl of 1X Perm/Wash Buffer (Cat. No 554723).